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Home > Products >Transcription Factor Antibodies :   Nuclear Receptor

NR1I3(nuclear receptor subfamily 1, group I, member 3) Antibody


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Catalog#   Accession # Size Price Qty
ARP45623_T100   NP_005113 100µg $229.00
Description: 
NR1I3 mediates the induction of transcription of cytochrome P450 (CYP) genes by phenobarbital (PB) and PB-type inducers. NR1I3 activation induces hepatic expression of detoxification enzymes and transporters and increases liver size. NR1I3 can also regulate both liver homeostasis and tumorigenesis in response to xenobiotic stresses.
Species Reactivity: 
Human  
Gene Symbol: 
NR1I3  
Alias Symbols: 
CAR; CAR1; MB67; MGC150433; MGC97144; MGC97209  
Peptide ID: 
P11906  
ELISA titre using peptide based assay: 
1:62500  
Search Keyword: 
NR1I3, NM_005122, NP_005113, Q5VTW5, NM_001077469, NP_001070937, NM_001077471, NP_001070939, NM_001077474, NP_001070942, NM_001077478, NP_001070946, NM_001077480, NP_001070948, NM_001077481, NP_001070949, NM_001077482, NP_001070950  
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Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human NR1I3 with an internal ID of P11906 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Ferguson,S.S., (2005) Mol. Pharmacol. 68 (3), 747-757 
Quality Control: 
Rabbit IgG purified by protein A chromatography. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 1.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP45623-QC13939-WB.jpg
Immunohistochemistry 1

ARP45623-QC13939-IHC.jpg
 
Protein Accession# Swissprot Id Nucleotide Accession# Protein Size Molecular Weight
NP_005113 Q5VTW5 NM_005122 348 39kDa
NP_001070937 NM_001077469 340 38kDa
NP_001070939 NM_001077471 309 35kDa
NP_001070942 NM_001077474 296 33kDa
NP_001070946 NM_001077478 339 38kDa
NP_001070948 NM_001077480 352 40kDa
NP_001070949 NM_001077481 314 36kDa
NP_001070950 NM_001077482 357 40kDa