Gene Symbol:
GFP
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Alias Symbols:
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Clone ID:
Full Length Antigen - cloneID 6E10
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ELISA titre using peptide based assay:
1:5000
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Search Keyword:
AAC53684, GFP, UU50974
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Click Here to view more information (protein size, molecular weight, etc)
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Species Reactivity:
(Primary - WB; Secondary - Computational Homology)
Bacterial
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Antigen Specificity:
Mouse monoclonal antibody produced in mouse immunized with E.coli derived full-length recombinant protein
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Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Key Reference:
Prendergast FG, et al., Biochemistry. 1978 Aug 22, 17(17): 3448-53
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Quality Control:
Mouse IgG purified by protein A chromatography.
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| Suggested starting concentrations are provided.
Optimal dilutions should be determined by end-user. Differences in calculated versus apparent
molecular weight may be due to post-translational modifications or protein hydrophobicity. For
research use only not for diagnostic, human, or veterinary use. |
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Western Blot
Full length GFP was expressed in E.coli as inclusion body and purified after protein refolding for mouse immunization. Clone 6E10 shows excellent western blot detection of when GFP-GST recombinant antigen was used for western blot test.
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Primary Antibodies by Class
Preview
