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Home > Products >  Antibodies
Home > Products >Antibodies :   Various
Home > Products >  Monoclonal Antibodies

Anti-E.coli NusA

Preview
Catalog#   Accession # Size Price Qty
AMM90036-1   ABJ02664 100µg $250.00
  Description: 
E.coli derived transcription elongation factor NusA, (54,8Kd), is a common used fusion partner due to its high solubility and popularity of pET vector technology.
Species Reactivity: 
Bacterial  
Gene Symbol: 
E.coli NusA  
Alias Symbols: 
 
Clone ID: 
Full Length Antigen - cloneID 3B1  
ELISA titre using peptide based assay: 
1:5000  
Search Keyword: 
ABJ02664, E.coli NusA, CP000468  
Click Here to view more information (protein size, molecular weight, etc)
Antigen Specificity: 
Mouse monoclonal antibody produced in mouse immunized with E.coli derived full-length NusA protein. 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Takeda, K., et al., Nature. 380 (6575), 627-630 (1996) 
Quality Control: 
Mouse IgG purified by protein A chromatography. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Full-length E, coli NusA (ABJ02664) was expressed in E.coli using pET-43 and purified for mouse immunization. Clone 3B1 shows excellent western blot detection of recombinant protein.
Immunoblot

AMM90036-1 3B1-WB.jpg
 
Protein Accession# Swissprot Id Nucleotide Accession# Protein Size Molecular Weight
ABJ02664 CP000468 495 55