NR0B1(nuclear receptor subfamily 0, group B, member 1) Antibody

• Catalog#: ARP45597_T100
• Accession#: NP_000466
• Size: 100µg

Description: 
NR0B1 is a protein that contains a DNA-binding domain. The protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in its gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

Species Reactivity:  Human   Gene Symbol:  NR0B1   Alias Symbols:  AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1   Peptide ID:  P11876   ELISA titre using peptide based assay:  1:312500   Search Keyword:  NR0B1, NM_000475, NP_000466, P51843   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human NR0B1 with an internal ID of P11876  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Mantovani,G., (2005) Fertil. Steril. 84 (5), 1542-1544  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 2.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP45597-QC13875-WB.jpg Immunohistochemistry 1 ARP45597-QC13875-IHC.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_000466	P51843	NM_000475	470	52kDa