ALOX15B(arachidonate 15-lipoxygenase, type B) Antibody

• Catalog#: ARP41895_T100
• Accession#: NP_001132
• Size: 100µg

Description: 
ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively.This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.

Species Reactivity:  Human   Gene Symbol:  ALOX15B   Alias Symbols:    Peptide ID:  P24432   ELISA titre using peptide based assay:  1:312500   Search Keyword:  ALOX15B, NM_001141, NP_001132, O15296, NM_001039130, NP_001034219, NM_001039131, NP_001034220   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ALOX15B with an internal ID of P24432  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Subbarayan,V., (2005) Neoplasia 7 (3), 280-293  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 2.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP41895-QC12526-WB.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_001132	O15296	NM_001141	676	74kDa
NP_001034219		NM_001039130	647	73kDa
NP_001034220		NM_001039131	602	67kDa