ANK1(ankyrin 1, erythrocytic) Antibody

• Catalog#: ARP42566_T100
• Accession#: NP_065211
• Size: 100µg

Description: 
Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known.Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Species Reactivity:  Human, Mouse, Rat, Dog   Gene Symbol:  ANK1   Alias Symbols:  ANK, SPH1, SPH2   Peptide ID:  P24804   ELISA titre using peptide based assay:  1:312500   Search Keyword:  ANK1, NM_020478, NP_065211, Q53ER1, NM_000037, NP_000028, NM_020479, NP_065212, NM_020481, NP_065214, P16157   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ANK1 with an internal ID of P24804  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Chang,T.L., (2004) Cell. Mol. Biol. (Noisy-le-grand) 50 (1), 59-66  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 1.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP42566-QC13040-WB.jpg Immunohistochemistry 1 ARP42566-QC13040-IHC-1.jpg Immunohistochemistry 2 ARP42566-QC13040-IHC-2.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_065211	Q53ER1	NM_020478	155	17kDa
NP_000028		NM_000037	1880	206kDa
NP_065212		NM_020479	133	15kDa
NP_065214	P16157	NM_020481	74	8kDa