TCF4(transcription factor 4) Antibody

• Catalog#: P100776_P050
• Accession#: NP_003190
• Size: 50µg

Description: 
TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. TCF4 is known to produce multiple transcripts; however as the complete structure is only known for the transcript that encodes the b isoform, that is the variant presented here.

Species Reactivity:  Human,Mouse,Rat,Dog   Gene Symbol:  TCF4   Alias Symbols:  E2-2, ITF2, SEF2, SEF2-1, SEF2-1A, SEF2-1B   Peptide ID:  P01845   ELISA titre using peptide based assay:  1:62500   Search Keyword:  TCF4(transcription factor 4) Antibody,NM_003199,NP_003190,P15884   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human TCF4 with an internal ID of P01845  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Raurell,I., (2006) J. Biol. Chem. 281 (3), 1401-1411  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot P100776-QC0776-WB.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_003190	P15884	NM_003199	667	71kDa