CHRNB2(cholinergic receptor, nicotinic, beta 2 (neuronal)) Antibody

• Catalog#: ARP34970_T100
• Accession#: NP_000739
• Size: 100µg

Description: 
Mutations in nAChRs are found in a rare form of nocturnal frontal lobe epilepsy . Previously, some nAChR mutations have been described that are associated with additional neurological features such as psychiatric disorders or cognitive defects. A new CHRNB2 mutation located in transmembrane region 3 (M3), outside the known ADNFLE mutation cluster. The CHRNB2 mutation I312M, which occurred de novo in twins, markedly increases the receptor's sensitivity to acetylcholine. Phenotypically, the mutation is associated not only with typical ADNFLE, but also with distinct deficits in memory. The cognitive problems are most obvious in tasks requiring the organization and storage of verbal information.

Species Reactivity:  Human   Gene Symbol:  CHRNB2   Alias Symbols:  EFNL3   Peptide ID:  P23870   ELISA titre using peptide based assay:  1:312500   Search Keyword:  CHRNB2(cholinergic receptor, nicotinic, beta 2 (neuronal)) Antibody,NM_000748,NP_000739,P17787   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CHRNB2 with an internal ID of P23870  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Hales,T.G., (2006) J. Biol. Chem. 281 (12), 8062-8071  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 2.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP34970-QC11821-WB.jpg Immunohistochemistry 1 ARP34970-QC11821-IHC.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_000739	P17787	NM_000748	502	57kDa