ADAT1(adenosine deaminase, tRNA-specific 1) Antibody

• Catalog#: ARP40718_P050
• Accession#: NP_036223
• Size: 50µg

Description: 
ADAT1 is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, the family participates in the pre-mRNA editing of nuclear transcripts. ADAT1, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA.This gene is a member of the ADAR (adenosine deaminase acting on RNA) family. Using site-specific adenosine modification, proteins encoded by these genes participate in the pre-mRNA editing of nuclear transcripts. The protein encoded by this gene, tRNA-specific adenosine deaminase 1, is responsible for the deamination of adenosine 37 to inosine in eukaryotic tRNA.

Species Reactivity:  Human   Gene Symbol:  ADAT1   Alias Symbols:  HADAT1   Peptide ID:  Y00970   ELISA titre using peptide based assay:  1:312500   Search Keyword:  ADAT1(adenosine deaminase, tRNA-specific 1) Antibody,NM_012091,NP_036223,Q9BUB4   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ADAT1 with an internal ID of Y00970  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Maas,S., (1999) Proc. Natl. Acad. Sci. U.S.A. 96 (16), 8895-8900  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP40718-QC9827-WB.jpg Immunohistochemistry 1 ARP40718-QC9827-IHC.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_036223	Q9BUB4	NM_012091	502	55kDa