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Home > Products >  Antibodies
Home > Products >Antibodies :   Cell Adhesion
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SPP1 Antibody

Preview
Catalog#   Accession # Size Price Qty
ARP36677_T100   NP_000573 100µg $198.00
  Description: 
The SPP1 gene encodes an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels. This protein also contributes to several steps in the process of prostate carcinogenesis and metastasis.
Species Reactivity: 
Human  
Gene Symbol: 
SPP1  
Alias_Symbols: 
HGNC:11255, BNSP, BSPI, ETA-1, MGC110940, OPN  
Peptide ID: 
P08104  
ELISA titre using peptide based assay: 
1:1562500  
Click Here to view more information (protein size, molecular weight, etc)
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human SPP1 with an internal ID of P08104 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Iwata,M., et al., (2004) Blood 103 (12), 4496-4502 
Quality Control: 
Rabbit IgG purified by protein A chromatography. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 1.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP36677-QC5706-WB.jpg
Immunohistochemistry 1

ARP36677-QC5706-IHC.jpg
 
Protein_Accession# Protein Size Nucleotide Accession# Swissprot Id Molecular Weight
NP_000573 300 NM_000582 P10451-2 33kDa