GABPA (GA binding protein transcription factor, alpha subunit 60kDa) Antibody

• Catalog#: P100832_T100
• Accession#: NP_002031
• Size: 100µg

Description: 
GA Binding Protein . chain (GABP-. subunit, GABPA, nuclear respiratory factor-2 subunit . transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Species Reactivity:  Human,Mouse,Rat,Dog,ZebraFish   Gene Symbol:  GABPA   Alias_Symbols:  HGNC:4071, E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A   Peptide ID:  P01930   ELISA titre using peptide based assay:  1:312500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human GABPA with an internal ID of P01930.  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 200 ul of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Xue, H.H., et al., (2004) Nat.Immunol.5(10),1036-1044  Quality Control:  Rabbit total Ig G  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.5-1.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,003 as second antibody. Immunoblot P100832-QC0832-WB.jpg Immunohistochemistry 1 P100832-QC0832-IHC-1.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_002031	454	NM_002040	Q06546	51kDa
XP_379849	878			97kDa