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Home > Products >  Antibodies
Home > Products >Antibodies :   RNA Binding Proteins

CPSF3 (cleavage and polyadenylation specific factor 3, 73kDa) Antibody

Preview
Catalog#   Accession # Size Price Qty
ARP40840_T100   NP_057291 100µg $198.00
  Description: 
CPSF3 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF3 subfamily. It is component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition.
Species Reactivity: 
Human,Mouse,Rat,Dog,ZebraFish  
Gene Symbol: 
CPSF3  
Alias_Symbols: 
CPSF, CPSF-73, CPSF73  
Peptide ID: 
Y01092  
ELISA titre using peptide based assay: 
1:312500  
Click Here to view more information (protein size, molecular weight, etc)
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CPSF3 with an internal ID of Y01092 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Calzado,M.A., (2004) J. Virol. 78 (13), 6846-6854 
Quality Control: 
Rabbit IgG purified by protein A chromatography. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 5.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP40840-QC9900-WB.jpg
 
Protein_Accession# Protein Size Nucleotide Accession# Swissprot Id Molecular Weight
NP_057291 684 NM_016207 Q9UKF6 75kDa