Home > Products >  Antibodies
Home > Products >Antibodies :   RNA Binding Proteins

CARF Antibody


Preview
Catalog#   Accession # Size Price Qty
ARP40869_T100   NP_060102 100µg $229.00
Description: 
CARF was first cloned as a novel binding partner of ARF from a yeast-interactive screen. CARF and ARF colocalize in the perinucleolar region and have a collaborative function. In the nucleoplasm, CARF interacts with p53 and enhances its function. The p53 downregulates CARF in a negative feedback regulatory loop and may also involve p53 antagonist HDM2.
Species Reactivity: 
Human,Mouse,Rat,Dog  
Gene Symbol: 
CARF  
Alias_Symbols: 
FLJ20036  
Peptide ID: 
Y01104  
ELISA titre using peptide based assay: 
1:312500  
Click Here to view more information (protein size, molecular weight, etc)
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CARF with an internal ID of Y01104 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Hasan,M.K., (2002) J. Biol. Chem. 277 (40), 37765-37770 
Quality Control: 
Rabbit IgG purified by protein A chromatography. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 0.3125µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

ARP40869-QC10143-WB.jpg
Immunohistochemistry 1

ARP40869-QC10143-IHC.jpg
 
Protein_Accession# Protein Size Nucleotide Accession# Swissprot Id Molecular Weight
NP_060102 580 NM_017632 Q9NXV6 64kDa