AATF (apoptosis antagonizing transcription factor) Antibody

Preview

Catalog#

Accession #

Size

Price

Qty

ARP38958_T100

NP_036270

100µg

$229.00

Description:
The AATF gene encodes a protein that was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Gene Symbol:
AATF

Alias_Symbols:
CHE-1, CHE1, DED

Peptide ID:
Y00767

ELISA titre using peptide based assay:
1:312500

Click Here to view more information (protein size, molecular weight, etc)

Species Reactivity:
(Primary - WB; Secondary - Computational Homology)
Human,Mouse,Rat,Dog

Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human AATF with an internal ID of Y00767

Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Key Reference:
Lehner,B. (2004) Genome Res. 14 (7), 1315-1323

Quality Control:
Rabbit IgG purified by protein A chromatography.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot
Suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.

Immunoblot

ARP38958-QC8973-WB.jpg

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_036270	560	NM_012138	Q9NY61	63kDa