CDK8 (cyclin-dependent kinase 8) Antibody

• Catalog#: AVARP03050_T100
• Accession#: NP_001251
• Size: 100µg

Description: 
The protein encoded by CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Species Reactivity:  Human,Mouse,Rat,Dog,ZebraFish   Gene Symbol:  CDK8   Alias_Symbols:  HGNC:1779, K35   Peptide ID:  P01020   ELISA titre using peptide based assay:  1:12500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CDK8 with an internal ID of P01020  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Akoulitchev,S., et al., (2000) Nature 407 (6800), 102-106  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 1.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot AVARP03050-QC3700-WB.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_001251	464	NM_001260	P49336	53kDa