ALF (TFIIA-alpha/beta-like factor) Antibody

• Catalog#: ARP32837_T100
• Accession#: NP_751946
• Size: 100µg

Description: 
The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.

Species Reactivity:  Human   Gene Symbol:  ALF   Alias_Symbols:  MGC26254   Peptide ID:  P03857   ELISA titre using peptide based assay:  1:1562500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ALF with an internal ID of P03857  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Upadhyaya,A.B., et al., (2002) J. Biol. Chem. 277(37), 34208-16  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 1.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP32837-QC4904-WB.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_751946	453	NM_172196		50kDa