LEF1 (lymphoid enhancer-binding factor 1) Antibody

• Catalog#: ARP32403_T100
• Accession#: NP_057353
• Size: 100µg

Description: 
Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1.

Species Reactivity:  Human, Mouse, Rat, Dog, ZebraFish   Gene Symbol:  LEF1   Alias_Symbols:  HGNC:6551, DKFZp586H0919, TCF1ALPHA   Peptide ID:  P03394   ELISA titre using peptide based assay:  1:62500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human LEF1 with an internal ID of P03394.  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Liu,X., et al., (2004) J. Invest. Dermatol. 123 (2), 264-274  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 2.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP32403-QC1875-WB.jpg Immunohistochemistry 1 ARP32403-QC1875-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_057353	399	NM_016269	Q9UJU2	44 kDa