TP53 (tumor protein p53 (Li-Fraumeni syndrome)) Antibody

• Catalog#: AVARP02055_T100
• Accession#: NP_000537
• Size: 100µg

Description: 
TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process.

Species Reactivity:  Human   Gene Symbol:  TP53   Alias_Symbols:  HGNC:11998, Cys51Stop, TRP53, p53   Peptide ID:  P01288   ELISA titre using peptide based assay:  1:62500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human p53 with an internal ID of P01288.  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Zakut-Houri, R., et al., (1985) EMBO J. 4: 1251-1255.  Quality Control:  Rabbit Ig G is purified by Protein G/A affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_000537	393	NM_000546	P04637	44 kDa