CEBPA (CCAAT/enhancer binding protein (C/EBP), alpha) Antibody

• Catalog#: P100908_P050
• Accession#: NP_004355
• Size: 50µg

Description: 
CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. It has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Species Reactivity:  Human   Gene Symbol:  CEBPA   Alias_Symbols:  HGNC:1833, C/EBP-alpha, CEBP   Peptide ID:  P02005 (QC#10353 &QC#0908)   ELISA titre using peptide based assay:  1:312500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CEBPA with an internal ID of P02005  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Zhu,Q.S., et al., (2004) J. Biol. Chem. 279 (29), 29902-29910  Quality Control:  Rabbit Ig G is purified by the peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 5.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot P100908-QC0908-WB.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_004355	358	NM_004364	P49715	38kDa