E2F5 (E2F transcription factor 5, p130-binding) Antibody

• Catalog#: P100920_P050
• Accession#: NP_001942
• Size: 50µg

Description: 
E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

Species Reactivity:  Human   Gene Symbol:  E2F5   Alias_Symbols:  HGNC:3119, E2F-5   Peptide ID:  P02022   ELISA titre using peptide based assay:  1:1562500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human E2F5 with an internal ID of P02022.  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Ohtani,N., et al., (2003) J. Cell Biol. 162 (2), 173-183  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.4µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot P100920-QC0920-WB.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_001942	346	NM_001951	Q15329	38 kDa