APEX1 Antibody

• Catalog#: ARP37014_T100
• Accession#: NP_033817
• Size: 100µg

Description: 
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells.

Species Reactivity:  Mouse,Human   Gene Symbol:  APEX1   Alias_Symbols:  APE, Apex, HAP1, Ref-1   Peptide ID:  P09925   ELISA titre using peptide based assay:  1:62500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Mouse Apex1 with an internal ID of P09925  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Izumi,T., et al., (2005) Proc. Natl. Acad. Sci. U.S.A. 102 (16), 5739-5743  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 2.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP37014-QC6780-WB.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_033817	317	NM_009687	Q544Z7	35kDa