GJB1 (gap junction protein, beta 1, 32kDa) Antibody

• Catalog#: ARP36600_T100
• Accession#: NP_000157
• Size: 100µg

Description: 
This gene encodes for a Connexin 32 protein. A large Charcot-Marie-Tooth disease family has been identified with a novel mutation in the Cx32 P2 promoter region at position -526bp. Cx32 mutants that are associated with a CNS phenotype may have toxic effects in oligodendrocytes.

Species Reactivity:  Human,Mouse,Rat,Dog   Gene Symbol:  GJB1   Alias_Symbols:  HGNC:4283, CMTX, CMTX1, CX32   Peptide ID:  P07839   ELISA titre using peptide based assay:  1:312500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human GJB1 with an internal ID of P07839  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Dagli,M.L., et al., (2004) Carcinogenesis 25 (4), 483-492  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 2.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP36600-QC5550-WB.jpg Immunohistochemistry 1 ARP36600-QC5550-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_000157	283	NM_000166	P08034	31kDa