ASCL1 (achaete-scute complex homolog 1 (Drosophila)) Antibody

• Catalog#: ARP32355_T100
• Accession#: NP_004307
• Size: 100µg

Description: 
ASCL1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers.

Species Reactivity:  Human   Gene Symbol:  ASCL1   Alias_Symbols:  HGNC:738, ASH1, HASH1, MASH1   Peptide ID:  P03344   ELISA titre using peptide based assay:  1:312500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ASCL1 with an internal ID of P03344  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Somasundaram,K., et al., (2005) Oncogene. 24(47), 7073-83  Quality Control:  Rabbit IgG purified by protein A chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 1.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP32355-QC1814-WB.jpg Immunohistochemistry 1 ARP32355-QC1814-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_004307	236	NM_004316	P50553	25kDa