ANP32A (acidic (leucine-rich) nuclear phosphoprotein 32 family, member A) Antibody

• Catalog#: ARP40203_P050
• Accession#: NP_006296
• Size: 50µg

Description: 
ANP32A is a novel potent heat-stable inhibitor protein of protein phosphatase 2A. It may play a key role in self-renewing cell populations where it may act in the nucleus to limit their sensitivity to transformation. Being a tumor suppressor, ANP32A represses cell growth through inhibition of transcription by blocking acetylation and phosphorylation of histone H3 and initiating its proapoptotic activity.

Species Reactivity:  Human   Gene Symbol:  ANP32A   Alias_Symbols:  C15orf1, I1PP2A, LANP, MAPM, MGC119787, MGC150373, PHAP1, PHAPI, PP32   Peptide ID:  S01612   ELISA titre using peptide based assay:  1:312500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ANP32A with an internal ID of S01612  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Stelzl,U., (2005) Cell 122 (6), 957-968  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP40203-QC9984-WB.jpg Immunohistochemistry 1 ARP40203-QC9984-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_006296	249	NM_006305	P39687	29kDa