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Home > Products >  Antibodies
Home > Products >Antibodies :   Protein Kinases
Home > Products >Antibodies :   Transcription Regulators
Home > Products >  Transcription Factor Antibodies

GTF2F1 (general transcription factor IIF, polypeptide 1, 74kDa) Antibody

Preview
Catalog#   Accession # Size Price Qty
P100810_P050   NP_002087 50µg $260.00
  Description: 
GTF2F1 is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
Species Reactivity: 
Human,Dog  
Gene Symbol: 
GTF2F1  
Alias_Symbols: 
BTF4, RAP74, TF2F1, TFIIF  
Peptide ID: 
P01891  
ELISA titre using peptide based assay: 
1:312500  
Click Here to view more information (protein size, molecular weight, etc)
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human GTF2F1 with an internal ID of P01891 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Zhang,C., et al., (2005) Mol. Cell. Biol. 25 (9), 3583-3595 
Quality Control: 
Rabbit Ig G is purified by peptide affinity chromatography method. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

P100810-QC0810-WB.jpg
 
Protein_Accession# Protein Size Nucleotide Accession# Swissprot Id Molecular Weight
NP_002087 517 NM_002096 P35269 58kDa