CLCNKB(chloride channel Kb) Antibody

• Catalog#: ARP34916_P050
• Accession#: AAH20873
• Size: 50µg

Description: 
Chloride channel Kb (CLCNKB) is a member of the CLC family of voltage-gated chloride channels, which comprises at least 9 mammalian chloride channels. Each is believed to have 12 transmembrane domains and intracellular N and C termini. Mutations in CLCNKB result in the autosomal recessive Type III Bartter Syndrome. CLCNKA and CLCNKB are closely related (94% sequence identity), tightly linked (separated by 11 kb of genomic sequence) and are both expressed in mammalian kidney.

Species Reactivity:  Human   Gene Symbol:  CLCNKB   Alias Symbols:  RP11-5P18.8, CLCKB, ClC-K2, MGC24087, hClC-Kb   Peptide ID:  P06140   ELISA titre using peptide based assay:  1:312500   Search Keyword:  CLCNKB, AAH20873, Q8WW53, NM_000085, NP_000076, P51801   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CLCNKB with an internal ID of P06140  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  N/A  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP34916-QC9577-WB.jpg Immunohistochemistry 1 ARP34916-QC9577-IHC.jpg Immunohistochemistry 2 ARP34916-QC9577-IHC2.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
AAH20873	Q8WW53		462	50kDa
NP_000076	P51801	NM_000085	687	75kDa