CACNB4(calcium channel, voltage-dependent, beta 4 subunit) Antibody

• Catalog#: ARP34959_P050
• Accession#: NP_001005747
• Size: 50µg

Description: 
CACNB4 is a member of the beta subunit family, a protein in the voltage-dependent calcium channel complex. CACNB4 plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. This gene encodes a member of the beta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Species Reactivity:  Human   Gene Symbol:  CACNB4   Alias Symbols:  CAB4; CACNLB4; EA5; EJM   Peptide ID:  P06182   ELISA titre using peptide based assay:  1:312500   Search Keyword:  CACNB4, NM_001005747, NP_001005747, O00305-2   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CACNB4 with an internal ID of P06182  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Kobayashi,T., (2007) Biochem. Biophys. Res. Commun. 360 (3), 679-683  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP34959-QC18260-WB.jpg

 

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_001005747	O00305-2	NM_001005747	486	55kDa