AIRE(autoimmune regulator) Antibody

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Catalog#

Accession #

Size

Price

Qty

ARP32650_P050

NP_000374

50µg

$289.00

Description:
The function of the protein encoded by the AIRE gene is not well defined. However it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Gene Symbol:
AIRE

Alias Symbols:
AIRE1; APECED; APS1; APSI; PGA1

Peptide ID:
P03660/QC2086 & QC7399

ELISA titre using peptide based assay:
1:62500

Search Keyword:
AIRE, NM_000383, NP_000374, O43918

Click Here to view more information (protein size, molecular weight, etc)

Species Reactivity:
(Primary - WB; Secondary - Computational Homology)
Human,Mouse,Rat

Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human AIRE with an internal ID of P03660

Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Key Reference:
Ilmarinen,T., (2008) Mol. Immunol. 45 (7), 1847-1862

Quality Control:
Rabbit Ig G is purified by peptide affinity chromatography method.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot
Suggested dilution at 0.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.

Immunoblot

ARP32650-QC7399-WB.jpg

Protein Accession#	Swissprot Id	Nucleotide Accession#	Protein Size	Molecular Weight
NP_000374	O43918	NM_000383	545	58kDa