BRCA1 (breast cancer 1, early onset) Antibody

• Catalog#: AVARP02017_P050
• Accession#: NP_009229
• Size: 50µg

Description: 
BRCA1, which functions as a tumor suppressor in human breast cancer cells, is a nuclear phosphoprotein which associates with RNA polymerase II holoenzyme. Mutations in BRCA1 are predicted to be responsible for approximately 45% of inherited breast cancer and more than 80% of inherited breast and ovarian cancer. BRCA1 may function as a transcriptional regulator, due to an amino terminal DNA-binding ring finger motif, nuclear localization signals, and an acidic carboxy terminal domain. BRCA1 is also a granin-like protein that functions as a secreted growth inhibitory protein. BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth. This function is compromised in breast cancer either by direct mutation or by alterations in gene expression.

Species Reactivity:  Human   Gene Symbol:  BRCA1   Alias_Symbols:  HGNC:1100, BRCAI, IRIS, PSCP, RNF53   Peptide ID:  P01017   ELISA titre using peptide based assay:  1:62500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human BRCA1 with an internal ID of P01017  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Mark,W.Y., et al., (2005) J. Mol. Biol. 345 (2), 275-287  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot AVARP02017-QC0144-WB.jpg Immunohistochemistry 1 AVARP02017-QC0144-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_009229	680	NM_007298		76kDa
NP_009225	1863	NM_007294		208kDa
NP_009226	1863	NM_007295		208kDa
NP_009227	1863	NM_007296		208kDa
NP_009230	1624	NM_007299		181kDa
NP_009231	1598	NM_007300		178kDa
NP_009232	1496	NM_007301		166kDa
NP_009233	1822	NM_007302		203kDa
NP_009234	721	NM_007303		81kDa
NP_009235	759	NM_007304		85kDa
NP_009236	719	NM_007305		81kDa
NP_009237	59	NM_007306	P38398	7kDa