CXCL12 (chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)) Antibody

• Catalog#: AVARP07031_P050
• Accession#: NP_000600
• Size: 50µg

Description: 
Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF, or IL1. The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.

Species Reactivity:  Human   Gene Symbol:  CXCL12   Alias_Symbols:  HGNC:10672, PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1, chemokine   Peptide ID:  P01478   ELISA titre using peptide based assay:  1:62500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CXCL12 with an internal ID of P01478  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Sadir,R., et al., (2004) J. Biol. Chem. 279 (42), 43854-43860  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot AVARP07031-QC0514-WB.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_000600	93	NM_000609	P48061	11kDa