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Home > Products >  Antibodies
Home > Products >Antibodies :   Cell Cycle Proteins
Home > Products >Antibodies :   Transcription Regulators
Home > Products >  Transcription Factor Antibodies

ATF5 (activating transcription factor 5) Antibody

Preview
Catalog#   Accession # Size Price Qty
P100654_P050   NP_036200 50µg $260.00
  Description: 
Overexpression of the bZIP protein ATF5, a transcriptional activator, stimulates asparagine synthetase promoter/reporter gene transcription via the nutrient-sensing response unit. ATF5 is also a new interacting partner of cyclin D3.
Species Reactivity: 
Human  
Gene Symbol: 
ATF5  
Alias_Symbols: 
HGNC:790, ATFX, HMFN0395  
Peptide ID: 
P01702  
ELISA titre using peptide based assay: 
1:62500  
Click Here to view more information (protein size, molecular weight, etc)
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ATF5 with an internal ID of P01702 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Liu,W., et al., (2004) Biochem. Biophys. Res. Commun. 321 (4), 954-960 
Quality Control: 
Rabbit Ig G is purified by peptide affinity chromatography method. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 0.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

P100654-QC0654-WB.jpg
Immunohistochemistry 1

P100654-QC0654-IHC.jpg
 
Protein_Accession# Protein Size Nucleotide Accession# Swissprot Id Molecular Weight
NP_036200 282 NM_012068 Q9Y2D1 31kDa