CDC2 (cell division cycle 2, G1 to S and G2 to M) Antibody

• Catalog#: AVARP03024_P050
• Accession#: NP_001777
• Size: 50µg

Description: 
The protein encoded by CDC2 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.

Species Reactivity:  Human   Gene Symbol:  CDC2   Alias_Symbols:  HGNC:1722, CDK1   Peptide ID:  P00438   ELISA titre using peptide based assay:  1:12500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CDC2 with an internal ID of P00438  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Kramer,A., et al., (2004) Nat. Cell Biol. 6 (9), 884-891  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot AVARP03024-QC0106-WB.jpg Immunohistochemistry 1 AVARP03024-QC0106-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_001777	297	NM_001786	P06493	34kDa