CHEK1 (CHK1 checkpoint homolog (S. pombe)) Antibody

• Catalog#: ARP32589_P050
• Accession#: NP_001265
• Size: 50µg

Description: 
CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.

Species Reactivity:  Human,Mouse,Rat,Dog   Gene Symbol:  CHEK1   Alias_Symbols:  HGNC:1925, CHK1   Peptide ID:  P03592   ELISA titre using peptide based assay:  1:312500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CHEK1 with an internal ID of P03592  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Kramer,A., et al., (2004) Nat. Cell Biol. 6 (9), 884-891  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP32589-QC2258-WB.jpg Immunohistochemistry 1 ARP32589-QC2258-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_001265	476	NM_001274	O14757	54kDa