CHRND (cholinergic receptor, nicotinic, delta) Antibody

• Catalog#: AVARP13022_P050
• Accession#: NP_000742
• Size: 50µg

Description: 
The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma and delta subunits. After acetylcholine binding, the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Species Reactivity:  Human,Dog   Gene Symbol:  CHRND   Alias_Symbols:  HGNC:1965, ACHRD, CMS2A, FCCMS, SCCMS   Peptide ID:  P01336   ELISA titre using peptide based assay:  1:62500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human CHRND with an internal ID of P01336  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Engel,A.G., et al., (1996) Hum. Mol. Genet. 5 (9), 1217-1227  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 1.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot AVARP13022-QC0397-WB.jpg Immunohistochemistry 1 AVARP13022-QC0397-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_000742	517	NM_000751	Q07001	59 kDa