Clone Number:
2D9
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Species Reactivity:
Rabbit
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ELISA titre using peptide based assay:
1:125000
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Antigen Specificity:
A mouse monoclonal antibody ( 2D9 ) raised against Rabbit IgG Heavy Chain
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Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Key Reference:
McCormack, WT, et al., (1983) Hybridoma 2(1):97-107
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Quality Control:
Mouse IgG is purified by protein CA-AS
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| Suggested starting concentrations are provided.
Optimal dilutions should be determined by end-user. Differences in calculated versus apparent
molecular weight may be due to post-translational modifications or protein hydrophobicity. For
research use only not for diagnostic, human, or veterinary use. |
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Western Blot
Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
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Immunoblot
AMM90030-16-2D9-WB.jpg
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Preview
