Human p53

• Catalog#: A902005P_100
• Accession#: NP_000537
• Size: 100µg

Description: 
p53 is an important DNA binding protein that is frequently mutated in cancer. p53 has several functions including: tumor suppression, DNA repair, and recombination. Recombinant p53 was expressed in E. coli and purified from inclusion bodies. This protein is devoid of any post-translational modifications and suitable for use as a substrate. Activity: Active in DNA binding assays. Purity: ≥95% by SDS-PAGE.

Species:  Human   Gene Symbol:  TP53   Gene Aliases:  HGNC:11998, Cys51Stop, TRP53, p53

Storage:  Liquid. In 20 mM Tris, pH 8.0, 0.6 M NaCl, 50% glycerol. For long term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.  Key Reference:  Matlashewski, G., et al., (1984) EMBO J. 3(13):3257-3262  Quality Control:  Active in DNA binding assay. Purity was found to be ≥95% by SDS-PAGE. Packaged in 1mg/ml.  Applications:  1: Refolded and purified from E.coli inclusion bodies, this protein is an excellent substrate for kinase assays; 2: Highly specific binding to wild-type p53 DNA binding site, this protein is an excellent reagent for development of DNA based binding assay  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

SDS-PAGE of Human P53 A902005P_T100-050725 copy.jpg