HeLa Cell Lysates

• Catalog#: AHL006
• Size: 200 μg

Description: 
Control cell lysates from HeLa cells, human epithelial cells from cervical adenocarcinoma, were cultured in the presence of media containing 10% FCS. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na₃O₄, 1 mM PMSF, 1 μg/ml aprotinin, 1 μg/ml leupeptin, 1 μg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). 200 μg of cellular protein was mixed with the same volume of 2 X SDS-PAGE loading buffer.

Reconstitution and Storage: 
Lyophilized. Add 100 μl distilled H₂O to the sample. Final concentration is 2 mg/ml. Final buffer is 31 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue. For long term storage store at -20°C. Avoid freeze thaw cycles. 

Key_Reference: 
Macville, M., et al. (1999) Cancer Res. 59, 141-150 

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.