K562 Cell Lysates

• Catalog#: AHL003
• Size: 200 μg

Description: 
Control cell lysates from K562 cells, human bone marrow cells derived from from chronic myelogenous leukemia (CML), were cultured in the presence of media containing 10% FCS. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na₃O₄, 1 mM PMSF, 1 μg/ml aprotinin, 1 μg/ml leupeptin, 1 μg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). 200 μg of cellular protein was mixed with the same volume of 2 X SDS-PAGE loading buffer.

Reconstitution and Storage: 
Lyophilized. Add 100 μl distilled H₂O to the sample. Final concentration is 2 mg/ml. Final buffer is 31 mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue. For long term storage store at -20°C. Avoid freeze thaw cycles. 

Key_Reference: 
Koeffler, H.P., and Golde, D.W. (1980) Blood 56:344-350 

Quality_Control: 
35 μg of protein was loaded onto a Bio-Rad mini gel system and analyzed using 10% SDS-PAGE. Anti-Human AREB6 (Cat. No. P100657) (1:1000) was used for signal detection using an ECL-based immunoblot. 

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Quality Control Data

35 μg of protein was loaded onto a Bio-Rad mini gel system and analyzed using 10% SDS-PAGE. Anti-Human AREB6 (Cat. No. P100657) (1:1000) was used for signal detection using an ECL-based immunoblot.