CLDN1 (claudin 1) Antibody

• Catalog#: ARP33623_P050
• Accession#: NP_066924
• Size: 50µg

Description: 
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This protein, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.

Species Reactivity:  Human,Dog   Gene Symbol:  CLDN1   Alias_Symbols:  HGNC:2032, CLD1, SEMP1   Peptide ID:  P04679   ELISA titre using peptide based assay:  1:312500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human CLDN1 with an internal ID of P04679.  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Coyne,C.B., et al., (2003) Am. J. Physiol. Lung Cell Mol. Physiol. 285 (5), L1166-L1178  Quality Control:  Rabbit IgG is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 1.0-5.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP33623-QC2513-WB.jpg Immunohistochemistry 1 ARP33623-QC2513-IHC.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_066924	211	NM_021101	O95832	23kDa