Species Reactivity:
Human
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Gene Symbol:
EGR1
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Alias_Symbols:
HGNC:3238, AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
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Peptide ID:
P03222
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ELISA titre using peptide based assay:
1:62500
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Click Here to view more information (protein size, molecular weight, etc)
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Antigen Specificity:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human EGR1 with an internal ID of P03222.
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Reconstitution and Storage:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Key Reference:
M. Sharon Stack, et al., (2007) J Biol Chem 282:4924
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Quality Control:
Rabbit Ig G is purified by peptide affinity chromatography method.
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| Suggested starting concentrations are provided.
Optimal dilutions should be determined by end-user. Differences in calculated versus apparent
molecular weight may be due to post-translational modifications or protein hydrophobicity. For
research use only not for diagnostic, human, or veterinary use. |
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Western Blot
Suggested dilution at 1.0-5.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,037 as second antibody.
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Immunoblot
ARP32241-QC1711-WB.jpg
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Immunohistochemistry 1
ARP32241-QC1711-IHC-1.jpg
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Immunohistochemistry 2
ARP32241-QC1711-IHC-2.jpg
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Preview
