AEBP1 (AE binding protein 1) Antibody

• Catalog#: ARP31592_P050
• Accession#: NP_001120
• Size: 50µg

Description: 
The adipocyte enhancer binding protein 1 (AEBP1) is a transcriptional repressor with carboxypeptidase (CP) activity. This protein binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. AEBP1 may play a role in differentiated vascular smooth muscle cells.

Species Reactivity:  Human   Gene Symbol:  AEBP1   Alias_Symbols:  HGNC:303, ACLP   Peptide ID:  P02373   ELISA titre using peptide based assay:  1:1562500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human AEBP1 with an internal ID of P02373.  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Layne,M.D., et al., (1998) J.Biol.Chem.273(25),15654-15660  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 0.2-5.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,015 as second antibody. Immunoblot ARP31592-QC1149-WB.jpg Immunohistochemistry 1 ARP31592-QC1149-IHC-1.jpg Immunohistochemistry 2 ARP31592-QC1149-IHC-2.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_001120	1158	NM_001129	Q14113	131kDa