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Human Genomic DNA

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Catalog#   Accession # Size Price Qty
AVAHG0001   $100.00
Description: 
Genomic DNA from selected human whole Blood is purified by the method described in Sambrook et al. (1). Greater than 90% of the DNA is longer than 50 kb in size as measured by pulsed-field gel electrophoresis. The DNA is suitable for Southern blot hybridizations, genomic analysis (including PCR), and genomic library construction. Human Genomic DNA comes from multiple anonymous donors.
Source: 
Human Whole Blood  
Package Size: 
100 μg / 100 μl  
DNA Concentration: 
1 mg / ml in TE buffer  
Molecular Weight: 
Greater than 90% of the DNA is large than 50 kb in size, as measure by pulsed-filed gel electrophoresis followed by ethidium bromide staining.  
Storage Conditions: 
Store at 4°C. Do not freeze. Avoid agitation. 
Key Reference: 
Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 9.14 
Spectrophotometric Analysis: 
A 50 μl sample of human Genomic DNA is added to 0.95 ml of TE buffer and allowed to equilibrate for 16 hours at room temperature before spectrophotometric analysis. A 260 / A250 > 1.05; A260 / A 280 > 1.80 
Inhibition of Enzyme Activity: 
No inhibition of Taq polymerase enzyme activity is observed using 20 human promoter-specific oligo for 1 Kb PCR amplification in 100 μl reaction volume with 2 μl genomic DNA template 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.