5-Methylcytosine Antibody

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Catalog#

Accession #

Size

Price

Qty

AMM99021

50 µg

$180.00

Description:
Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure. Clone number 33D3 is useful in several applications including: ELISA (0.1 µg/ml), Flow Cytometry (1 µg/ml), Cryo Sections (5-10 µg/ml),Paraffin Sections (5-10 µg/ml), and RIA (0.1 µg/ml).

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Species Reactivity:
(Primary - WB; Secondary - Computational Homology)
Human

Antigen Specificity:
Mouse monoclonal antibody (33 D3) was made against 5-methylcytosine conjugated to ovalbumin.

Reconstitution and Storage:
Antibody at a concentration of 1 mg/ml in 10 mM phosphate buffer, 150 mM NaCl, pH 7.4. Store at -20°C. Avoid freeze thaw cycles.

Key Reference:
Maki, Gary K., et al., (2007) Biosensors and Bioelectronics (PMID: 17936611 )

Quality Control:
Mouse IgG1 is purified by Protein A affinity chromatography method.

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot

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