E2F4 (E2F transcription factor 4, p107/p130-binding) Antibody

• Catalog#: ARP31175_P050
• Accession#: NP_001941
• Size: 50µg

Description: 
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Species Reactivity:  Human,Mouse,Dog   Gene Symbol:  E2F4   Alias_Symbols:  HGNC:3118, E2F-4   Peptide ID:  P01918   ELISA titre using peptide based assay:  1:312500   Click Here to view more information (protein size, molecular weight, etc)

Antigen Specificity:  Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human E2F4 with an internal ID of P01918  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  DuPree,E.L., et al., (2004) Cancer Res. 64 (13), 4390-4393  Quality Control:  Rabbit Ig G is purified by peptide affinity chromatography method.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Western Blot Suggested dilution at 2.0/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. Immunoblot ARP31175-QC0826WB.jpg

 

Protein_Accession#	Protein Size	Nucleotide Accession#	Swissprot Id	Molecular Weight
NP_001941	413	NM_001950	Q16254	44 kDa