Anti-HbxAg

• Catalog#: AVAMM2005
• Size: 200 µg

Description: 
The hepatitis B virus (HBV) protein HBx has been reported to induce liver cancer in transgenic mice and transactivates a variety of viral and cellular promoters. HBx protein is detected early and frequently in hepatitis B infection. HBx is able to bind to singlestranded DNA and RNA as well as several cellular proteins including p53 and other transcription factors.

Clone Number:  4H9

Antigen Specificity:  Mouse monoclonal antibody (4H9) raised against a recombinant HBxAg  Reconstitution and Storage:  Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Ekaterina Dadachova, et al., (2007) PLoS Genet 10:e1114  Quality Control:  Mouse IgG is purified by protein G affinity chromatography  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Immunoblot example: AVAMM2005-4H3-WB.jpg Western Blot Suggested working concentration is 0.01 - 0.1 µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-mouse IgG should be diluted in 1: 50,000 - 100,000 as second antibody.