Anti-Transferrin

• Catalog#: AVAMM00004-4
• Size: 200 µg

Description: 
Transferrin is a plasma protein which transports iron. Transferrin has two irom binding sites and serves as the source of iron for hemoglobin synthesis. Transferrin has also been used as a targeting ligand for liposomes and anticancer drugs.

Species Reactivity:  Human   Clone Number:  7C1

Antigen Specificity:  Mouse monoclonal antibody (7C1) raised against human transferrin protein.  Reconstitution and Storage:  Lyophilized. Add 100 µl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles  Key Reference:  Aisen, P., (2004) Int. J. Biochem. Cell Biol. 36(11):2137-2143  Quality Control:  Mouse IgG2a is purified by protein A affinity chromatography.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Immunoblot example AVAMM00004-4 7C1-WB.jpg Western Blot Suggested dilution at 0.01-1 µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-mouse IgG should be diluted in 1: 50,000 - 100,000 as second antibody.