Anti-Amphetamine

• Catalog#: AVAMM16004-2
• Size: 200 µg

Description: 
Amphetamine (AMPH) is a potent central nervous system (CNS) stimulant with additional peripheral sympathomimetic effects. AMPH has been used clinically in the treatment of obesity, minimal brain dysfunction, narcolepsy, depression and to counter fatigue and are also subjected to widespread abuse. Following the acute administration of the AMPHs there is an increase in blood pressure, heart rate, respiration, sweating and tremor. The behavioral manifestations include species specific stereotypic behavior, anorexia, sleeplessness, hyperexcitability, and hyperactivity. AMPH is a indirect agonists, causing the release of newly synthesized norepinephrine and dopamine and blocking the reuptake of these transmitters from the synapse, resulting to an increase in the concentration of catecholamines in the synapse as well as an overall increase in catecholaminergic activity in the brain.

Clone Number:  6F2   ELISA:  Suitable for use in ELISA assays at a 1:1000 dilution with HRP detection systems.

Antigen Specificity:  Mouse monoclonal antibody (6F2) was made against a BSA-amphetamine conjugate.  Reconstitution and Storage:  Lyophilized. Add 100 µl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Bowyer, J. F., et al., (2004) Neurotoxicology 25(4), 555-72  Quality Control:  Mouse IgG1 is purified by ammonium sulfate precipitation.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Applications and Examples: A competitive ELISA was performed using various drugs at a concentration of 10 ng/ml. Only amphetamine was able to block the binding of the antibody to the antigen.