Anti-Methamphetamine

• Catalog#: AVAMM16001-6
• Size: 200 µg

Description: 
Methamphetamine (METH) is closely related chemically to amphetamine (AMPH), but the central nervous system effects of METH are greater. METH is a potent central nervous system stimulant with additional peripheral sympathomimetic effects. METH and AMPH have been used clinically in the treatment of obesity, minimal brain dysfunction, narcolepsy, depression and to counter fatigue and are also subjected to widespread abuse. METH is indirect agonists, causing the release of newly synthesized norepinephrine and dopamine and blocking the reuptake of these transmitters from the synapse, which can lead to an increase in the concentration of catecholamines in the synapse as well as an overall increase in catecholaminergic activity in the brain. The mechanism of METH-induced neurotoxicity for all monoaminergic cell types may lie primarily with the dopaminergic system in the striatum and the interaction between METH-induced release of dopamine and its ability to inhibit monoamine oxidase.

Clone Number:  4E2   ELISA:  Suitable for use in ELISA assays at a 1:1000 dilution with HRP detection systems.

Antigen Specificity:  Mouse monoclonal antibody (4E2) was generated against a BSA-Methamphetamine conjugate.  Reconstitution and Storage:  Lyophilized. Add 100 µl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Beckett, A. H., et al., (1965) Nature 206(990), 1260-1  Quality Control:  Mouse IgG1 is purified by ammonium sulfate precipitation.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Applications and Examples: A competitive ELISA was performed using various drugs at a concentration of 10 ng/ml. Only methamphetamine was able to block the binding of the antibody to the antigen.