Anti-Morphine (3A6)

• Catalog#: AVAMM16006
• Size: 200 µg

Description: 
Morphine is thought to produce reinforcement phenomena via stimulation of μ, δ, and κ opioid receptors that regulate stress perception, pain control, reward behavior, and neurohormone secretion in reward-relevant brain systems. It has the highest affinity for µ, followed by δ and κ. Rapid activation of the μ-opioid receptor by morphine results in a euphoric phenotype, thus conferring the reinforcing effects of the drug. This activation is accompanied by extracellular dopamine release, which alters several events related to the cAMP signal transduction pathway. Of particular significance is that CREB seems to be modified by morphine, thereby affecting addictive behavioral phenomena, such as withdrawal symptoms. Suitable for use in ELISA assays.

Clone Number:  3A6   ELISA:  Suitable for use in ELISA assays at a 1:1000 dilution with HRP detection systems.

Antigen Specificity:  Mouse monoclonal antibody (3A6) was made against a BSA-morphine conjugate.  Reconstitution and Storage:  Lyophilized. Add 100 µl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.  Key Reference:  Bowyer, J. F., et al., (2004) Neurotoxicology 25(4), 555-72; Iwata, S, et al., 1997, Synapse 26(3),281-91  Quality Control:  Mouse IgG1 is purified by ammonium sulfate precipitation.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Applications and Examples: A competitive ELISA was performed using various drugs at a concentration of 10 ng/ml. Only morphine was able to block the binding of the antibody to the antigen.