Anti-MBP

• Catalog#: AVAMM25001
• Size: 200 µg

Description: 
The maltose binding protein (MBP) from E. coli is a commonly used fusion partner for recombinant protein expression. MBP has been reported to enhance the solubility of aggregation prone proteins. Its affinity for maltodextrins allows easy purification via amylose affinity chromatography.

Species Reactivity:  E. coli   Clone Number:  4C6

Antigen Specificity:  A mouse monoclonal antibody (4C6) raised against a recombinant E. coli MBP fusion protein.  Reconstitution and Storage:  Lyophilized. Add 100 µl of distilled water. Final antibody concentration is 2 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles  Key Reference:  Fox, J.D., et al., (2001) Protein Sci. 10:622-630  Quality Control:  Mouse IgG is purified by ammonium sulfate precipitation.  Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.

Immunoblot AVAMM25001-2 1D4-WB.jpg Western Blot Suggested dilution at 1 µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-mouse IgG should be diluted in 1: 50,000 - 100,000 as second antibody.